This study investigates the interplay between Manufacturing ambidexterity, Industry 4.0 readiness, and sustainable Excellence in Egypt's food and beverage sector. It explores how Industry 4.0 readiness mediates and moderates the relationship between ambidexterity and sustainability outcomes. A quantitative research design was employed, utilizing a survey of 308 professionals in Egypt's food and beverage industry. Structural equation modeling (SEM) was used to analyze the relationships among Manufacturing ambidexterity, Industry 4.0 readiness, and sustainable Excellence. The results reveal that Industry 4.0 readiness fully mediates and significantly moderates the relationship between Manufacturing ambidexterity and sustainable Excellence. While Manufacturing ambidexterity alone does not directly impact sustainable Excellence, its effect becomes significant through Industry 4.0 readiness, highlighting the importance of digital transformation. This study focuses on a single sector in Egypt, limiting generalizability. Future research could explore other industries and regions or examine specific dimensions of Industry 4.0 readiness. The findings emphasize the need for organizations to invest in digital infrastructure and foster ambidextrous capabilities to achieve sustainability goals. Policymakers are encouraged to support Industry 4.0 adoption through incentives and training programs to enhance competitiveness and sustainability in emerging markets. This study contributes to the limited research on the application of Manufacturing ambidexterity and Industry 4.0 technologies in developing economies, offering insights into achieving sustainable Excellence through digital transformation.